Verhoeff’s Stain
By Dr S. Das
Reagents and Preparation • Solution A: 5 grams of hematoxylin dissolve in 100 ml of absolute alcohol on a hot plate, cool, and filter. • …
Hale’s Dialyzed Iron Technique
Reagents and Preparation • Dialyzed Iron Pour the FeCl3 solution into the boiling distilled water and stir well. The solution will turn dark red when ...
Toluidine Blue
Reagents and Preparation 1 gram toluidine blue in 100 ml of 50% isopropanol Staining Procedure 1) Deparaffinize the slide by heating in hot incubation. 2) ...
Masson’s Trichrome Stain
Reagents and Preparation of Stain Solution A Solution B Solution C Staining Procedure 1) Deparaffinize the slide by heating in hot incubation. 2) To remove ...
Van Gieson Stain
Reagents Prepare fresh picric acid-fuchsin solution before staining. To prepare the solution, take 9 drops of saturated picric acid in a test tube. Add 1 ...
Reticulin Stain
Reagents To make a silver solution, the following reagents are required: Make the titrated silver solution in a test tube freshly before each & every ...
Iron Stain (Perl’s Stain)
Reagents The working solution is to be made by pouring two stock solutions in equal volume. Should be prepared freshly before each staining procedure. Staining ...
Hematoxylin and Eosin Stain
Reagents Preparation of Stain Mix the hematoxylin powder in ethanol and shake well. Heat the distilled water, add the alum to it and boil it ...