Non-Specific Esterase (NSE) Staining
Principle Leucocytes esterase are enzymes that hydrolyze acyl or chroacyl esters of alpha-napthol. There are nine isoenzymes. Five isoenzymes are stained specifically with napthol AS-D ...
Periodic Acid Schiff (PAS) Staining
Principle: 1-2 glycol groups are oxidised by periodic acid to produce dialdehydes. The dialdehydes, when exposed to leucobasic fuchsin (Schiff’s reagent), give positive reactions in ...
Sudan Black B Staining
Principle Sudan black B is a lipophilic dye that binds irreversibly to an undefined granule component in granulocytes, eosinophils, and some monocytes. It cannot be ...
Bone Marrow Iron Stain
Principle The test is based on the principle of a positive Prussian blue reaction by iron particles. Non-haem iron reacts with potassium ferrocyanide to form ...
Staining of Peripheral Blood Smear
Romanowsky stains are used universally for routine staining of blood films. Principle The main components of Romanowsky stain are: 1) A cationic or basic dye ...
Preparation of Peripheral Blood Smear
The film is made from a drop of blood spread evenly on a slide and stained. It is important to ensure that the film is ...