Manual Reticulocyte Count
By Dr S. Das
Principle Reticulocytes are immature red blood cells (RBCs) that contain residual ribosomal RNA (rRNA). These RNA remnants react with basic dyes like azure B, brilliant …
Activated Partial Thromboplastin Time (APTT)
Purpose The Activated Partial Thromboplastin Time (APTT) assay is useful for three reasons: as a screening test for inherited or acquired deficiencies of the intrinsic ...
ABO blood grouping
Cell and Serum Testing The procedure describe the method of detection of ABO antigens on the red cells and reciprocal antibodies in the serum (Land ...
Prothrombin Time
Five different components are involved in normal hemostasis namely (blood vessels, platelets, plasma coagulation factors, their inhibitors, and the fibrinolytic system). The cornerstone assays for ...
Bleeding Time
Five different components are involved in normal hemostasis, namely blood vessels, platelets, plasma coagulation factors, their inhibitors, and the fibrinolytic system. Blood vessels and platelets ...
Sucrose Lysis Test
Principle This is a screening test for paroxysmal nocturnal hemoglobinuria (PNH) based on the examination of red cell fragility. This test is based on the ...
Osmotic Fragility Test: Diagnosis of Hereditary Spherocytosis
Principle The osmotic fragility test gives an indication of the surface area / volume ratio of erythrocytes. The ability of the normal red cell to ...
Test for Screening of G6PD Deficiency (Methaemoglobin Reduction Test)
Principle G6PD is the first enzyme in the HMP shunt pathway, which protects red cells from any oxidant injury by providing source of NADPH. G6PD ...
Sickling Test
Principle This test detect the presence of HbS depend on the decreased solubility of this haemoglobin at low oxygen tension. In deoxygenated state, insoluble crystals ...
Direct Coombs Test
Principle Detection of (in vivo) sensitised red cells with IgG or complement component C3b & C3d by use of anti-human globulin in the form of ...